The tips are also derivatized and ready for use in specific biomolecular applications. Fortebio s octet red96 system is a multifunctional, labelfree, realtime analysis instrument. North america, conference, boston, ma may 04 may 08, 2020 more than 2,800 worldrenowned experts, visionaries, and influencers from top pharma, biotech, academic, and government institutions convene in boston each spring for pegs. Fortebio s octet qk, biorads proteon xpr36, and ge healthcares biacore 3000. After 180 s online loading of purified antibody 1 mab 1, 100 nm, each sensor was soaked in pbsf for 20 min to achieve equilibrium. The fortebio octet system is currently used by both pharmaceutical and biotechnology companies in applications ranging from target validation to process monitoring. New graphical display options to thicken sensor traces and customize axis scale. Fortebio s octet red384 and htx blibased instruments are suitable for supernatant screening in that its non flowbased platform has no fluidics that can become clogged. Antihigg fc sensor or protein a sensor igg standard purified sample, cell culture supernatant, crude lysate, etc.
The data analysis ht software is the recommended choice for all new users. Priced for startup academic and biotech labs, low volume users are no longer bound by the tradeoff between cost and. Analysis ht software is not available in the octet cfr software suite installer. Biolayer interferometry bli is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. Various molecules can be attached to the surface, and multiple layers can be bound to study a myriad of biological interactions. Many sensor data tend to drift eg, because of temperature. The biosensor tips are coated with a proprietary biocompatible matrix that is uniform, nondenaturing, and has minimal nonspecific binding. Measure binding affinities over a range of biomolecular interactions, determine concentrations of engineered proteins or contaminating impurities the octet provides a wealth of information to enable. The octet data analysis ht software is designed for fast analysis of large datasets from quantitation, kinetic and epitope binning assays. The sensors can be loaded with samples offline, allowing for longer capture times of the antibody to the sensor. The blitz system package consists of the blitz instrument, the blitz pro software cd, power cord, usb cable, blitz quickstart guide, and a starter kit figure 11.
Fortebio is a business unit of molecular devices, offering products that span multiple technology vectors including analytical instrumentation and software, clone picking and imaging, and. With the new amine reactive biosensors, customers can analyze a wider range of kinetic interactions by directly coupling the target molecule of their choice using familiar techniques. Whats new in the octet system data acquisition software, release 7. Fortebio provides integrated solutions for early stage biologics development. Bli experiments are used to determine the kinetics and affinity of molecular interactions. The octet qke has provided us with a robust and versatile platform for the measurement of antibody antigen interactions. All octet instruments provide sample temperature control from 4c above ambient temperature up to 40c. Launches new octet r red96 system and dip and readtm protein l biosensor read this article along with other. Since its sensors are disposable and do not involve the handling of liquid samples, the prospect of cross contamination is virtually eliminated, thereby enabling parallel antibody screening applications. Fortebio introduces high precision octet system biosensor. Ar2g sensors were activated and quenched in advance. This is the equilibrium binding level req there is a fixed amount of ligand on the sensor surface, so there must be a maximum possible amount of sample binding at equilibrium. Pall fortebio s octet data acquisition and data analysis software is rapidly evolving to better address the growing applications and needs of our customers. The direct quantitation assay format of the protein a biosensor provides elisa data in less than 2 minutes and can be automated for high throughput screening efforts.
The octet software kinetics analysis and reporting. Fortebio octet data acquisition and data analysis software is rapidly evolving to better address the growing applications and needs of our customers. Ahc sensors proved unreliable for kinetic or binning assays at times. For questions or to get started, please contact unit director alexey tomilov, phd, at. We describe three assay orientations that we term in tandem, premix, and classical sandwich blocking, and we perform each of them on three platforms. To examine if the sa sites are exposed on each sensor type, a biotinylated antigen kda, degree of labeling biotin. Fortebio s octet red384, and wasatch microfluidicss ibis mx96. Whats new in the octet system data analysis software, release 7. Added ability to exclude full sensor trace from analysis. Protein g biosensor for use on companys octet instruments.
Strengthens portfolio with new biosensors for research and development published. Part 1 is a system introduction providing information about blitz system features and bli assay technology. Octet red384 getting started introduction load analyte sample. The instrument is very robust, easy to use and is well supported by an experienced support staff of application scientists, software engineers, service engineers and sales. The blitz system enables realtime quantitation of solutionbased analytes or kinetic char acterization of molecular interactions. The biacore t100 is equipped with the most sensitive sensor that allows the study of interaction of even small compounds, such as small molecule inhibitors and peptides. These comparison tables provide a general guide to the features included in various versions of the software. Fortebios octet red384 and htx blibased instruments are suitable for. An online tool for the realtime kinetic analysis of binding. Measure binding affinities over a range of biomolecular interactions, determine concentrations of engineered proteins or contaminating impurities the octet provides a wealth of information to enable more informed decision making. Realtime, labelfree bio analytics for determination of. Compatible with octet and blitz platforms ninta nta biosensors taking advantage of the widespread use of polyhistidine protein tags histag in the biopharmaceutical industry, the ninta biosensor further facilitates easy detection and rapid characterization of histagged molecules.
Developing a robust quantitation assay for monoclonal. Developing robust quantitation assays on the octet platform 2 application note 2 3 affords a dynamic range of. Separate binding graphs are displayed based on the sensor type figure. The octet platform based on biolayer interferometry bli technology is a whole set of system including instruments, biosensors, reagents and assay kits to support the evaluation of biomolecular interactions in 96 or 384well microplates. The octer red 384 obtains the best lymphocytes at an acceptable cost because it provides lots of cell data bound to the antigen antibody ratio in a short time. Biolayer interferometry bli center for macromolecular. Converting an elisa assay into an octet quantitation assay. Understanding fortebios sensors for highthroughput. Fortebio biosensors are coated with a proprietary biocompatible matrix that is uniform and nondenaturing with minimal nonspecific binding. The kinetic assay was performed with a baseline 60 s in pbsf. Fortebio is a business unit of molecular devices llc offering products that include labelfree analytical instrumentation and software, clone picking and ima. Offering both high quality data and high throughput analysis, the octet platform allows you to more thoroughly characterize candidate molecules without extending timelines. Fortebios protein a biosensors enable rapid antibody quantitation from crude lysates, cell culture supernatants and complex matrices.
We demonstrate the use of labelfree realtime optical biosensors in competitive binding assays by epitope binning a panel of antibodies. Specify the well locations of the active sensors and the associated samples. Comparison of biosensor platforms in the evaluation of high affinity antibodyantigen binding kinetics. Octet cfr software provide kinetics and quantitation analysis of antibodies, proteins, protein residual contaminants and other biological molecules. The sensors can be loaded with samples offline, allowing for longer capture times of the antibody to the sensor, especially. The octet platform includes instruments, biosensors, reagents and assay kits for biomolecular interactions analysis in microplates. Octet realtime drug and protein binding kinetics unit.
Fortebio offers 21 cfr part 11 software and a full line of gxp products and services as part of the octet red96e gxp package. Comparison of biosensor platforms in the evaluation of. The octet platform consists of 8 and 16channel instruments that accommodate 96 and 384well assay formats. Integration of the instrument into an automation system was completely supported by fortebio. Octet cfr software octet software comparison user friendly software for data acquisition and analysis. New biosensors to increase flexibility of octet system. Our technology platforms encompass workflows from antibody discovery and cell line development through to biomolecular analysis of the biologic product. Creative biolabs proudly provides you the octet system from fortebio for measuring antibody affinity.
The octet red96e system is widely used in analytical core labs for protein structurefunction studies and small molecule screening campaigns. Exploring blocking assays using octet, proteon, and. Most curves, if the binding is left for long enough, will reach a point where the rates of association and dissociation are the same. The tips are derivatized and ready for use in a diverse set of biomolecular applications. The equipment is suitable for obtaining cells that produce antibodies, selected by a sensors capable of producing fast results. Octet platform there the biosensor comes to the sample. Biacore t100, interspots on the array proteon xpr36 and ibis mx96, and ahc sensor tips octet red384 that were immobilized with capture reagent without any ligand. Spr label free and biosensor instruments biacore t200 and. In a bli experiment, one molecule is immobilized to a dip and read biosensor and binding to a second. Reagents for use with fortebio molecular interactions. The fortebio octet system includes the octet instrument and octet software.
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